.. _backbone-label: ###### Trgt ###### ************** Introduction ************** TRGT is a tool for targeted genotyping of tandem repeats from PacBio HiFi data. In addition to the basic size genotyping, TRGT profiles sequence composition, mosaicism, and CpG methylation of each analyzed repeat and visualization of reads overlapping the repeats. ********** Versions ********** - 1.1.0 ********** Commands ********** - trgt ************* Example job ************* Adjust slurm options based on job requirements (`slurm cheat sheet `_): .. code:: #!/bin/bash #SBATCH -p partitionName # batch, gpu, preempt, mpi or your group's own partition #SBATCH -t 1:00:00 # Runtime limit (D-HH:MM:SS) #SBATCH -N 1 # Number of nodes #SBATCH -n 1 # Number of tasks per node #SBATCH -c 4 # Number of CPU cores per task #SBATCH --mem=8G # Memory required per node #SBATCH --job-name=trgt # Job name #SBATCH --mail-type=FAIL,BEGIN,END # Send an email when job fails, begins, and finishes #SBATCH --mail-user=your.email@tufts.edu # Email address for notifications #SBATCH --error=%x-%J-%u.err # Standard error file: --.err #SBATCH --output=%x-%J-%u.out # Standard output file: --.out module purge ### Optional, but highly recommended. module load trgt/XXXX ### Latest version is recommended.