.. _backbone-label: ################## Nf-core-scrnaseq ################## ************** Introduction ************** nf-core/scrnaseq is a bioinformatics best-practice analysis pipeline for processing 10x Genomics single-cell RNA-seq data. ********** Versions ********** - 2.5.1 - 2.7.0 - 2.7.1 ********** Commands ********** - scrnaseq ************* Example job ************* Adjust slurm options based on job requirements (`slurm cheat sheet `_): .. code:: #!/bin/bash #SBATCH -p partitionName # batch, gpu, preempt, mpi or your group's own partition #SBATCH -t 1:00:00 # Runtime limit (D-HH:MM:SS) #SBATCH -N 1 # Number of nodes #SBATCH -n 1 # Number of tasks per node #SBATCH -c 4 # Number of CPU cores per task #SBATCH --mem=8G # Memory required per node #SBATCH --job-name=nf-core-scrnaseq # Job name #SBATCH --mail-type=FAIL,BEGIN,END # Send an email when job fails, begins, and finishes #SBATCH --mail-user=your.email@tufts.edu # Email address for notifications #SBATCH --error=%x-%J-%u.err # Standard error file: --.err #SBATCH --output=%x-%J-%u.out # Standard output file: --.out module purge ### Optional, but highly recommended. module load nf-core-scrnaseq/XXXX ### Latest version is recommended.